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KMID : 0353319960200020265
Oral Biology Research
1996 Volume.20 No. 2 p.265 ~ p.280
Genotype and Allele frequency of the Short tandem repeat F13A01 locus by polymerase chain reaction in Korean


Abstract
Allelic frequency and genotype distribution of short tandem repeat(STR) F13A01 locus was analysed by polymerase chain reaction, polyacrylamide gel electrophoresis and silver staining from human genomic deoxyribonucleic acid(DNA) was extracted from 205 unrelated Korean to be applied to forensic identification and parentage testing as a database. The results were as follows
1. 5 alleles and 11 genotypes of F13A01 locus were detected and heterozygosity value was 62.0% and the observed each alleles and allelic frequency was 3.2(0.363), 4(0.105), 5(0.063), 6(0.466), 16(0.002).
2. The allelic diversity value was 0.639 and the power of discrimination was 0.804.
3. Compared with observed number of alleles and allele frequency in ethnic difference, result was appeared to be similar to that of Japanese and Asians, while was appeared to be much different to that of Blacks and Caucasians in the observed number of alleles and frequency of allele 3.2, 5, 7.
From the above result of this investigation, the allelic frequency of STR F13A01 locus in the Korean was coy isiderd to be useful for individual identification and parentage testing as a database.
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